Abstract
In vitro culturing of endometrial cells obtained from the uterine mucosa or ectopic sites is used to study molecular and cellular signalling relevant to physiologic and pathologic reproductive conditions. However, the lack of consensus on standard operating procedures (SOPs) for deriving, characterising and maintaining primary cells in two- or three-dimensional cultures from eutopic or ectopic endometrium may be hindering progress in this area of research. Guidance for unbiased in vitro research methodologies in the field of reproductive science remains essential to increase confidence in the reliability of in vitro models. We present herein the protocol for a Delphi process to develop a consensus on in vitro methodologies using endometrial cells (ENDOCELL Project). A steering committee composed of leading scientists will select critical methodologies, topics and items that need to be harmonised and that will be included in a Survey. An enlarged panel of experts (ENDOCELL Working Group) will be invited to patriciate in the Survey and provide their ratings to the items to be harmonised. According to Delphi, an iterative investigation method will be adopted. Recommended measures will be finalised by the steering committee. The study received full ethical approval from the Ethical Committee of the Maastricht University (ref. FHML-REC/2021/103). The study findings will be available in both peer-reviewed articles and will also be disseminated to appropriate audiences at relevant conferences.
| Original language | English |
|---|---|
| Pages (from-to) | G1-G8 |
| Number of pages | 8 |
| Journal | Reproduction and Fertility |
| Volume | 3 |
| Issue number | 3 |
| Early online date | 1 Jul 2022 |
| DOIs | |
| Publication status | Published - Jul 2022 |
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10.1530/RAF-22-0041Licence: CC BY-NC-ND
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Romano, A., Guo, S.-W., Brosens, J., Fazleabas, A. T., Gargett, C., Giselbrecht, S., Goette, M., Griffith, L., Taylor, H. S., Taylor, R. N., Vankelecom, H., Chapron, C., Chang, X., Khan, K. N., & Vigano', P. (2022). ENDOCELL- Seud: a Delphi protocol to harmonise methods in endometrial cell culturing. Reproduction and Fertility, 3(3), G1-G8. https://doi.org/10.1530/RAF-22-0041
Romano, Andrea ; Guo, Sun-Wei ; Brosens, Jan et al. / ENDOCELL- Seud : a Delphi protocol to harmonise methods in endometrial cell culturing. In: Reproduction and Fertility. 2022 ; Vol. 3, No. 3. pp. G1-G8.
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title = "ENDOCELL- Seud: a Delphi protocol to harmonise methods in endometrial cell culturing",
abstract = "In vitro culturing of endometrial cells obtained from the uterine mucosa or ectopic sites is used to study molecular and cellular signalling relevant to physiologic and pathologic reproductive conditions. However, the lack of consensus on standard operating procedures (SOPs) for deriving, characterising and maintaining primary cells in two- or three-dimensional cultures from eutopic or ectopic endometrium may be hindering progress in this area of research. Guidance for unbiased in vitro research methodologies in the field of reproductive science remains essential to increase confidence in the reliability of in vitro models. We present herein the protocol for a Delphi process to develop a consensus on in vitro methodologies using endometrial cells (ENDOCELL Project). A steering committee composed of leading scientists will select critical methodologies, topics and items that need to be harmonised and that will be included in a Survey. An enlarged panel of experts (ENDOCELL Working Group) will be invited to patriciate in the Survey and provide their ratings to the items to be harmonised. According to Delphi, an iterative investigation method will be adopted. Recommended measures will be finalised by the steering committee. The study received full ethical approval from the Ethical Committee of the Maastricht University (ref. FHML-REC/2021/103). The study findings will be available in both peer-reviewed articles and will also be disseminated to appropriate audiences at relevant conferences.",
author = "Andrea Romano and Sun-Wei Guo and Jan Brosens and Fazleabas, {Asgerally T} and Caroline Gargett and Stefan Giselbrecht and Martin Goette and Linda Griffith and Taylor, {Hugh S} and Taylor, {Robert N} and Hugo Vankelecom and Charles Chapron and Xiaohong Chang and Khan, {Khaleque N} and Paola Vigano'",
note = "Publisher Copyright: {\textcopyright} 2022 The authors Published by Bioscientifica Ltd.",
year = "2022",
month = jul,
doi = "10.1530/RAF-22-0041",
language = "English",
volume = "3",
pages = "G1--G8",
journal = "Reproduction and Fertility",
issn = "2633-8386",
publisher = "BioScientifica Ltd.",
number = "3",
}
Romano, A, Guo, S-W, Brosens, J, Fazleabas, AT, Gargett, C, Giselbrecht, S, Goette, M, Griffith, L, Taylor, HS, Taylor, RN, Vankelecom, H, Chapron, C, Chang, X, Khan, KN & Vigano', P 2022, 'ENDOCELL- Seud: a Delphi protocol to harmonise methods in endometrial cell culturing', Reproduction and Fertility, vol. 3, no. 3, pp. G1-G8. https://doi.org/10.1530/RAF-22-0041
ENDOCELL- Seud: a Delphi protocol to harmonise methods in endometrial cell culturing. / Romano, Andrea; Guo, Sun-Wei; Brosens, Jan et al.
In: Reproduction and Fertility, Vol. 3, No. 3, 07.2022, p. G1-G8.
Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - ENDOCELL- Seud
T2 - a Delphi protocol to harmonise methods in endometrial cell culturing
AU - Romano, Andrea
AU - Guo, Sun-Wei
AU - Brosens, Jan
AU - Fazleabas, Asgerally T
AU - Gargett, Caroline
AU - Giselbrecht, Stefan
AU - Goette, Martin
AU - Griffith, Linda
AU - Taylor, Hugh S
AU - Taylor, Robert N
AU - Vankelecom, Hugo
AU - Chapron, Charles
AU - Chang, Xiaohong
AU - Khan, Khaleque N
AU - Vigano', Paola
N1 - Publisher Copyright:© 2022 The authors Published by Bioscientifica Ltd.
PY - 2022/7
Y1 - 2022/7
N2 - In vitro culturing of endometrial cells obtained from the uterine mucosa or ectopic sites is used to study molecular and cellular signalling relevant to physiologic and pathologic reproductive conditions. However, the lack of consensus on standard operating procedures (SOPs) for deriving, characterising and maintaining primary cells in two- or three-dimensional cultures from eutopic or ectopic endometrium may be hindering progress in this area of research. Guidance for unbiased in vitro research methodologies in the field of reproductive science remains essential to increase confidence in the reliability of in vitro models. We present herein the protocol for a Delphi process to develop a consensus on in vitro methodologies using endometrial cells (ENDOCELL Project). A steering committee composed of leading scientists will select critical methodologies, topics and items that need to be harmonised and that will be included in a Survey. An enlarged panel of experts (ENDOCELL Working Group) will be invited to patriciate in the Survey and provide their ratings to the items to be harmonised. According to Delphi, an iterative investigation method will be adopted. Recommended measures will be finalised by the steering committee. The study received full ethical approval from the Ethical Committee of the Maastricht University (ref. FHML-REC/2021/103). The study findings will be available in both peer-reviewed articles and will also be disseminated to appropriate audiences at relevant conferences.
AB - In vitro culturing of endometrial cells obtained from the uterine mucosa or ectopic sites is used to study molecular and cellular signalling relevant to physiologic and pathologic reproductive conditions. However, the lack of consensus on standard operating procedures (SOPs) for deriving, characterising and maintaining primary cells in two- or three-dimensional cultures from eutopic or ectopic endometrium may be hindering progress in this area of research. Guidance for unbiased in vitro research methodologies in the field of reproductive science remains essential to increase confidence in the reliability of in vitro models. We present herein the protocol for a Delphi process to develop a consensus on in vitro methodologies using endometrial cells (ENDOCELL Project). A steering committee composed of leading scientists will select critical methodologies, topics and items that need to be harmonised and that will be included in a Survey. An enlarged panel of experts (ENDOCELL Working Group) will be invited to patriciate in the Survey and provide their ratings to the items to be harmonised. According to Delphi, an iterative investigation method will be adopted. Recommended measures will be finalised by the steering committee. The study received full ethical approval from the Ethical Committee of the Maastricht University (ref. FHML-REC/2021/103). The study findings will be available in both peer-reviewed articles and will also be disseminated to appropriate audiences at relevant conferences.
U2 - 10.1530/RAF-22-0041
DO - 10.1530/RAF-22-0041
M3 - Article
C2 - 35972317
SN - 2633-8386
VL - 3
SP - G1-G8
JO - Reproduction and Fertility
JF - Reproduction and Fertility
IS - 3
ER -
Romano A, Guo SW, Brosens J, Fazleabas AT, Gargett C, Giselbrecht S et al. ENDOCELL- Seud: a Delphi protocol to harmonise methods in endometrial cell culturing. Reproduction and Fertility. 2022 Jul;3(3):G1-G8. Epub 2022 Jul 1. doi: 10.1530/RAF-22-0041
